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The main component of dairy products is protein, which has an important influence on the physicochemical properties of dairy products. There are many methods to determine protein. The most basic and most commonly used method is to determine total nitrogen first and then multiply the protein coefficient of different foods. That is, the content of protein in foods. National standards for determining total nitrogen still use the Kjeldahl method. The method has good numerical accuracy and reproducibility, but the testing procedure is cumbersome and takes a long time, which is not suitable for real-time monitoring on the enterprise site. For the processing of the sample, it is generally necessary to use a digester for digestion.
Therefore, some instrument companies developed the Kjeldahl meter to determine the protein content. Due to the small amount of sample during the measurement, the digestion rate is greatly improved compared to the traditional method. In particular, the amount of steam generated during the distillation of the instrument is large, and the distillation rate is also higher than the conventional method. It is much faster, and the data detected by the instrument is not much different from the traditional methods. Therefore, many companies, even some inspection and supervision agencies, also use Kjeldahl determination of protein content. However, due to the high cost of the nitrogen determination instrument (imported equipment costs about 160,000 yuan or 200,000 yuan), small businesses generally cannot afford it, and the traditional method is also time consuming, which is not conducive to the monitoring of the production process. Therefore, how to find a method that is both time-saving, economical, and accurate is very urgent.
On heating, sulfuric acid decomposes into sulfite, water and oxygen. Organic matter is oxidized to carbon dioxide and water, and the ammonia nitrogen of the protein reacts with excess sulfuric acid to convert it into sulfate, which is distilled in an alkaline solution. Distilled ammonia was absorbed with boric acid and titrated with an acid standard solution. The improved method combines traditional classic methods with modern instrument methods to increase digestion speed and shorten the time required for measurement. This enables companies not only to quickly obtain product protein indicators, but also to save a large number of detection reagents and improve the company’s economy. benefit. This method is economical and practical and can meet the needs of the company.
Therefore, some instrument companies developed the Kjeldahl meter to determine the protein content. Due to the small amount of sample during the measurement, the digestion rate is greatly improved compared to the traditional method. In particular, the amount of steam generated during the distillation of the instrument is large, and the distillation rate is also higher than the conventional method. It is much faster, and the data detected by the instrument is not much different from the traditional methods. Therefore, many companies, even some inspection and supervision agencies, also use Kjeldahl determination of protein content. However, due to the high cost of the nitrogen determination instrument (imported equipment costs about 160,000 yuan or 200,000 yuan), small businesses generally cannot afford it, and the traditional method is also time consuming, which is not conducive to the monitoring of the production process. Therefore, how to find a method that is both time-saving, economical, and accurate is very urgent.
On heating, sulfuric acid decomposes into sulfite, water and oxygen. Organic matter is oxidized to carbon dioxide and water, and the ammonia nitrogen of the protein reacts with excess sulfuric acid to convert it into sulfate, which is distilled in an alkaline solution. Distilled ammonia was absorbed with boric acid and titrated with an acid standard solution. The improved method combines traditional classic methods with modern instrument methods to increase digestion speed and shorten the time required for measurement. This enables companies not only to quickly obtain product protein indicators, but also to save a large number of detection reagents and improve the company’s economy. benefit. This method is economical and practical and can meet the needs of the company.
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